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The vertex scalp often responds better than the frontal scalp, possibly due to differences in blood flow and follicular sensitivity

Methylmalonic acid (MMA) and homocysteine are secondary markers that reflect true B12 metabolic activity

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For purification of His-tagged proteins, the supernatant was loaded onto a Ni-NTA column (GE Healthcare Life Sciences, PA) followed by washing with five column volumes of 20 mM imidazole and subsequent elution with 250 mM imidazole in the presence of 0.05% (w/v) DDM and 5% glycerol (w/v)

It supports liver detox pathways, helping eliminate toxins that often contribute to dull, tired-looking skin