For example, if you tried dissolving in PBS and got clumps, the phosphate could be precipitating with something or the salt could be causing aggregation try pure water or a dilute acetic acid instead
Landi, Persistent Symptoms in Patients After Acute COVID-19
After the washing step the R6G dye of the internalized non-targeted or targeted nanocarriers in living hECs was imaged using the 543 nm laser line on a Leica TCS SP5 confocal laser scanning microscope equipped with a sample holder kept at 37 C
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