Furthermore, treatment of mice with acetaminophen, but not AMAP, induces expression of the heat shock proteins hsp25 and hsp70 (Salminen et al
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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)
Although peripheral effects of the estradiol-GLP-1 conjugates are anticipated, most evidence suggests that the conjugate acts centrally to suppress food-reward (250)