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coli cells expressing GST fusion protein in pGEX vector Isopropyl-1-thio--D-galactopyranoside (IPTG), 100 mM stock 2.3 Affinity purification of GST fusion protein Glutathione Sepharose 4B bulk matrix (GE Healthcare or Sigma) (see Note 3) Column (BioRad 2.5 cm 10 cm Econo-Column) 10 mM glutathione buffer: 50 mM Tris, 10 mM reduced glutathione, pH 8.0 (make fresh daily) PBS/EDTA/PMSF: 1X PBS, 5 mM EDTA, 0.15 mM PMSF, pH 7.4 Pelleted E

[DOI] [PubMed] [Google Scholar] 64.Sen C.K., Packer L

V.et al (2012)

J Inherit Metab Dis 44:13931408

It helps regulate the production of white blood cells, which fight infections and diseases