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Gating residues involved in the occluded-to-inward conformational transition of OCTN2 Alternating-access transport by secondary active transporters requires the coordination of gates that enclose or preclude access to the substrate-binding site from either side of the membrane 51

After ultrasonic homogenization and a freeze/thaw cycle, the samples were centrifuged at 4 C, 13000 rpm, for 10 minutes

10.1016/j.fertnstert.2015.07.005 42