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Although hydrolyzed FB1 (HFB1) is not toxic, the C2 amino group can be acylated by fatty acids in vivo to generate a ceramide synthase inhibitor that is 10-times more toxic than FB1 in mammalian HT-29 cells (Seiferlein et al., 2007)

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JAMA Netw Open 2022;5:e2231982.ArticlePubMedPMC 9

Particularly, genes involved in lipid metabolism ( Abcg5 ), drug metabolism ( Cyp2a4 ), GSH metabolism ( Gstm2 and Gstm6 ), and oxidative phosphorylation ( Lhpp ) were expressed at higher levels in the liver of hepsin / mice compared with wild-type mice