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For GHK-Cu, this definitively verifies the peptide's primary sequence and, crucially, confirms the presence of the copper ion by matching the measured mass to the theoretical mass of the complex (~403.9 Da)

The supernatant and precipitate obtained were harvested by centrifugation at 14,200 g at 4 C for 10 min

4.2 Hyperthyroid-induced hepatic disease Hyperthyroid-induced hepatic disease involves liver injury caused by excessive thyroid hormones, resulting in hepatocellular degeneration, necrosis, and hepatomegaly (106), and often presents with jaundice, elevated transaminases, or cirrhosis (107)